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技術(shù)文章您當前的位置：首頁 > 技術(shù)文章 > 人鋅指蛋白RIZ(PRDM2)ELISA試劑盒?操作步驟

人鋅指蛋白RIZ(PRDM2)ELISA試劑盒?操作步驟

日期：2022-03-15瀏覽：1234次

人鋅指蛋白RIZ(PRDM2)ELISA試劑盒操作步驟:


1.  從室溫平衡20min后的鋁箔袋中取出所需板條，剩余板條用自封袋密封放回4℃。


2.  設置標準品孔和樣本孔，標準品孔各加不同濃度的標準品50μL；


3.  樣本孔中加入待測樣本50μL；空白孔不加。


4.  除空白孔外，標準品孔和樣本孔中每孔加入辣根過氧化物酶（HRP）標記的檢測抗體100μL，用封板膜封住反應孔，37℃水浴鍋或恒溫箱溫育60min。


5.  棄去液體，吸水紙上拍干，每孔加滿洗滌液（350μL），靜置1min，甩去洗滌液，吸水紙上拍干，如此重復洗板5次（也可用洗板機洗板）。


6.  每孔加入底物A、B各50μL，37℃避光孵育15min。


7.  每孔加入終止液50μL，15min內(nèi)，在450nm波長處測定各孔的OD值。


[實驗結(jié)果計算]


以所測標準品的OD值為橫坐標，標準品的濃度值為縱坐標，在坐標紙上或用相關(guān)軟件繪制標準曲線，并得到直線回歸方程，將樣品的OD值代入方程，計算出樣品的濃度。

蘇云金芽胞桿菌加拿大亞種 Bacillus thuringiensis subsp. canadensis

蘇云金芽胞桿菌科爾默亞種 Bacillus thuringiensis subsp. colmeri

蘇云金芽胞桿菌達爾可他亞種 Bacillus thuringiensis subsp. dakota

蘇云金芽胞桿菌達姆斯塔特亞種 Bacillus thuringiensis subsp. darmstadiensis

蘇云金芽胞桿菌松蜀亞種 Bacillus thuringiensis subsp. dendrolimus

大豆慢生根瘤菌 Bradyrhizobium japonicum

大豆慢生根瘤菌 Bradyrhizobium japonicum

大豆慢生根瘤菌 Bradyrhizobium japonicum

大豆慢生根瘤菌 Bradyrhizobium japonicum

大豆慢生根瘤菌 Bradyrhizobium japonicum

大豆慢生根瘤菌 Bradyrhizobium japonicum

大豆慢生根瘤菌 Bradyrhizobium japonicum

大豆慢生根瘤菌 Bradyrhizobium japonicum

大豆慢生根瘤菌 Bradyrhizobium japonicum

大豆慢生根瘤菌 Bradyrhizobium japonicum

大豆慢生根瘤菌 Bradyrhizobium japonicum

大豆慢生根瘤菌 Bradyrhizobium japonicum

大豆慢生根瘤菌 Bradyrhizobium japonicum

大豆慢生根瘤菌 Bradyrhizobium japonicum

大豆慢生根瘤菌 Bradyrhizobium japonicum

大豆慢生根瘤菌 Bradyrhizobium japonicum

大豆慢生根瘤菌 Bradyrhizobium japonicum

大豆慢生根瘤菌 Bradyrhizobium japonicum

大豆慢生根瘤菌 Bradyrhizobium japonicum

大豆慢生根瘤菌 Bradyrhizobium japonicum


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