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技術(shù)文章您當(dāng)前的位置:首頁 > 技術(shù)文章 > 人粘蛋白/粘液素5AC(MUC5AC)ELISA酶聯(lián)免疫試劑盒注意事項(xiàng)

人粘蛋白/粘液素5AC(MUC5AC)ELISA酶聯(lián)免疫試劑盒注意事項(xiàng)

日期:2022-02-10瀏覽:1309次

人粘蛋白/粘液素5AC(MUC5AC)ELISA酶聯(lián)免疫試劑盒注意事項(xiàng):


1. 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開封后如未用完,板條應(yīng)裝入密封袋中保存。


2. 濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果。


3. 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對(duì)其準(zhǔn)確性,以避免試驗(yàn)誤差。一次加樣時(shí)間最好控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排槍加樣。


4. 請(qǐng)每次測定的同時(shí)做標(biāo)準(zhǔn)曲線,最好做復(fù)孔。如標(biāo)本中待測物質(zhì)含量過高(樣本OD值大于標(biāo)準(zhǔn)品孔第一孔的OD值),請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)(n倍)后再測定,計(jì)算時(shí)請(qǐng)最后乘以總稀釋倍數(shù)(×n×5)。


5. 封板膜只限一次性使用,以避免交叉污染。


6. 底物請(qǐng)避光保存。


7. 嚴(yán)格按照說明書的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).


8. 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理。


9. 本試劑不同批號(hào)組分不得混用。


10. 如與英文說明書有異,以英文說明書為準(zhǔn)。


11.本公司產(chǎn)品僅用于科研。

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