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技術文章您當前的位置:首頁 > 技術文章 > 奧爾帕瓦約病毒PCR檢測試劑盒實驗規(guī)則

奧爾帕瓦約病毒PCR檢測試劑盒實驗規(guī)則

日期:2021-03-03瀏覽:1420次

奧爾帕瓦約病毒PCR檢測試劑盒實驗規(guī)則:

1、要保證移液槍的準確性,誤差不能超過2%??捎盟碗娮犹炱竭M行確定。但有專業(yè)人員進行矯正。
2、要配備20ul、50ul、100ul、1000ul和排槍各一支。吸取不同的液體后,要更換槍頭。即使是吸取標準品時。
3、要在實驗前1小時將試劑盒從冰箱中取出,使各種試劑都恢復到室溫,以使結果更穩(wěn)定。
4、實驗時,要使底物避光保存。
5、用槍吸取液體時速度不能太快,以免產(chǎn)生氣泡而使吸取量不準確。
6、吸取液體時,要用量程和需要量接近的槍去吸,減少誤差。
7、將液體加到酶標孔中時,避免槍頭和孔內液體接觸,可使槍頭上的液滴和孔壁接觸,液滴會自然流下去。
8、液體全部加完后,可將酶標板在桌子上平行輕輕晃動30秒,混勻液體。也可以用酶標儀的晃動功能。
9、應盡量做雙孔實驗,這樣才能保證數(shù)據(jù)的準確性。
10、對結果有疑問的樣品要用其它方法進行確證。

植物鈣調素(CAM)elisa試劑盒 Plant calmodulin, CAM Elisa Kit 
植物激素脫落酸(ABA )elisa試劑盒 Plant hormone abscisic acid, ABA Elisa Kit
植物維生素K1(VK1)elisa試劑盒 Plant Vitamin K1, VK1 Elisa Kit 
植物維生素B12(VB12)elisa試劑盒 Plant Vitamin B12, VB12 Elisa Kit 
植物維生素A(VA)elisa試劑盒 Plant Vitamin A, VA Elisa Kit 
植物維生素E(VE)elisa試劑盒 Plant Vitamin E, VE EElisa Kit 
植物維生素D2(VD2)elisa試劑盒 Plant Vitamin D2, VD2 Elisa Kit 
植物維生素D3(VD3)elisa試劑盒 Plant Vitamin D3, VD3 Elisa Kit 
植物維生素D(VD)elisa試劑盒 Plant Vitamin D, VD Elisa Kit 
植物維生素B6(VB6)elisa試劑盒 Plant Vitamin B6, VB6 Elisa Kit 
植物25羥基維生素D3(25(OH)D3/25 HVD3)elisa試劑盒 Plant 25-Dihydroxy vitamin D3, HVD3 Elisa Kit 
植物維生素C(VC)elisa試劑盒 Plant Human Vitamin C, VC Elisa Kit 
α-銀環(huán)蛇毒素(α-BGT)elisa試劑盒 α-bungarotoxin, α-BGT Elisa Kit
大腸桿菌宿主殘留蛋白(E.coli P)elisa試劑盒 Escherichia coli Protein, E.coli P Elisa Kit
 

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